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Objective To study the differentially expressed genes between keloids and normal control skin using cDNA microarray. Methods The PCR products of 8400 human genes were spotted on a chemical-material-coated-glass plate in array. Total RNAs were isolated from the freshly excised human keloids and normal control skin,and then were purified to mRNAs,which were reversely transcribed to cDNAs with the incorporations of fluorescent dUTP,for preparing the hybridization probes. The mixed probes were hybridized to the cDNA microarray. According to the results of cDNA microarray,three genes (NGF,TGF-?_ 1 and c-myc) were chosen to study their differential expression in keloids and normal control skin by RT-PCR. Results Among the 8400 target genes,there were 402 genes with different expression (4.75%),and they were mainly associated with extracellular matrix genes,cellular signal molecule genes and cellular skeleton molecule genes. Analysis of collagen related molecule and growth factor gene expression confirmed that our molecular data obtained by cDNA microarray were consistent with published biochemical and clinical observations of keloids. RT-PCR showed that the levels of gene expression of NGF,TGF-?_ 1 and c-myc were all higher in keloids than those in normal control skin. Conclusion Many genes were found to be involved in the formation of keloids. Further analysis of the obtained genes might contribute to reveal the molecular mechanism of keloids formation,in which NGF,TGF-?_ 1 and c-myc might play important roles.
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Objective To explore the change in gene expression of extracellular signal regulated protein kinase1 (erk1),erk2,p38MAPK and 3 c-Jun N- terminal kinases (jnk1,jnk2,jnk3) in fetal skin at different developmental stages and children skin. Methods After morphological characteristics of fetal skin at different developmental stages were examined histologically,gene expressions of erk1,erk2,p38MAPK,jnk1,jnk2 and jnk3 were determined with reverse transcription-polymerase chain reaction analysis (RT-PCR). Results Compared with early gestational fetal skin,the levels of gene expression of erk1 and erk2 showed no substantial change in late gestational fetal skin,while the contents of transcripts of p38MAPK and jnk1 were significantly decreased,the expressions of mRNA of jnk2 and jnk3 were obviously elevated. In children skin,gene expressions of erk2,p38MAPK and jnk1 were even more remarkably lowered. In contrary,gene expressions of jnk2 and jnk3 were marked enhanced. Conclusion The relative elevation of gene transcription of erk2 and p38MAPK and the inhibition of gene expression of jnk2 and jnk3 in fetal skin of earlier developmental stage might be related to fetal scarless healing.
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AIM: To investigate gene expression of bax, bcl-2 and p53 in fetal skin at different gestational ages and children skin in order to explore their potentially biological significance. METHODS: Apoptosis in skin specimens was determined by terminal deoxynucleotidy transferase mediated dUTP-biotin nick-end labeling technique (TUNEL). Gene expressions of bax, bcl-2 and p53 in skin at different developmental stages was examined with reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Along with fetal growth and development, the incidence rate of apoptosis was increased progressively in skin. In skin from early gestational fetus, bcl-2 was strongly expressed. This gene expression was progressively decreased with increment in gestational age. In children skin, the mRNA content of this gene was significantly reduced compared with fetal skin (P
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Objective To investigate gene expression of epidermal growth factor (EGF), its receptor (EGFR) and two protooncogenes (c-fos and c-myc), in fetal skin at different development stages and children skin and to delineate their potential biological significance. Methods Fetal skin biopsies of human embryo were obtained from spontaneous abortions at different gestational ages from 13 to 32 weeks and children skin specimens were collected from patients undergoing plastic surgery. After morphological characteristics of skin at different development stages were detected with histological methods, gene expressions of EGF, EGFR, c-fos and c-myc in skin at different development stages were examined with reverse transcription-polymerase chain reaction analysis (RT-PCR). Results Gene expression of EGF, EGFR, c-fos or c-myc could all be detected in fetal and childern skins. In fetal skin, the gene expression of these 4 genes was weak. Gene expression of these genes in skin was progressively enhanced with increasing gestational age. In children skin, the mRNA contents of these 4 genes were significantly increased in comparison with those in fetal skin (P